Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (4): 167-171.doi: 10.3969/j.issn.1004-616x.1999.04.004

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STUDY ON NUMERICAL CHROMOSOME ABERRATION IN HUMAN SPERM CELLS ON ACRYLONITRILE2EXPOSED WORKERS USING TWO2COLOR FLUORESCENCE IN SITU HYBRIDIZATION

Xu Dexiang1 , Zheng Lukang2 , Deng Lixia2   

  1. 1A nhui Medical University Department of Toxicology , Hef ei 　230032 　2 Sen Yat2sen University of Medical Science , Department of Toxicology , Guangz hou 　510089
  • Received:1998-09-29 Revised:1999-03-11 Online:1999-07-30 Published:1999-07-30

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Abstract: In this study , Fluorescence in situ hybridization (FISH) was used to investigate rates of sex chromosome aneuploidy in spermatozoa among 9 acrylonit rile2exposed workers and 9 healthy sperm donors. 74679 sperm nuclei were counted among 9 healthy sperm donors , and 91015 sperm nuclei among 9 acrylonit rileexposed workers. The average f requency of total chromosome numerical abnormalities was 0. 70 % in exposure group , and 0. 35 % in the cont rol. XY2bearing sperm was the most common sex chromosome disomy , with an average rate of 0. 37 % in exposure group , and 0. 20 % in the cont rol. XX2and YY2bearing sperm accounted for an additional 0. 10 % and 0. 23 % in exposure group , and 0. 05 % and 0. 10 % in the cont rol. Significantly increased f requencies of total sex chromosome disomy , and XX2 ,YY2and XY2bearing sperm were observed among acrylonit rile2exposed workers , as compared with healthy sperm donors. This study demonst rated that sperm FISH was a useful biomarker to detect and compare numerical chromosome aberration in human sperm cells for heritable risk assessment of acrylonit rile.

Key words: Aneuploidy, Sex Chromosome, Sperm, Fluorescence In Situ Hybridization (FISH), Acrylonit rile